Uniconazolewaa triazolenidaamiyaha koritaanka dhirtataas oo si weyn loo isticmaalo si loo habeeyo dhererka dhirta loogana hortago koritaanka iniinaha. Si kastaba ha ahaatee, habka molecular ee uniconazole uu u diidayo abuurka hypocotyl elongation weli ma cadda, waxaana jira daraasado yar oo kaliya oo isku daraya xogta transcriptome iyo metabolome si loo baaro habka dhererka hypocotyl. Halkan, waxaan ku aragnay in uniconazole si weyn u hakiyay elongation hypocotyl ee geedo kaabajka ubaxa Shiinaha. Waxa xiiso leh, oo ku salaysan falanqaynta transcriptome iyo metabolome la isku daray, waxaan ogaanay in uniconazole ay si weyn u saamaysay dariiqa "phenylpropanoid biosynthesis". Dariiqan, hal hidde-side oo ka mid ah qoyska hidde-sidayaasha nidaaminta enzyme, BrPAL4, oo ku lug leh lignin biosynthesis, ayaa si weyn hoos loogu dhigay. Intaa waxaa dheer, imtixaannada hal-isku-dhafan iyo laba-isku-dhafan ee khamiirka ah waxay muujiyeen in BrbZIP39 ay si toos ah ugu xidhi karto gobolka dhiirrigeliye ee BrPAL4 oo uu dhaqaajiyo qoraalkiisa. Nidaamka aamusinta hidda-wadaha fayraska uu keenay ayaa sii caddeeyay in BrbZIP39 uu si wanaagsan u xakameyn karo dhererka hypocotyl ee kaabajka Shiinaha iyo isku-dhafka hypocotyl lignin. Natiijooyinka daraasaddan ayaa siinaya fikrado cusub oo ku saabsan habka nidaaminta molecular ee cloconazole ee xakameynaya dhererka hypocotyl ee kaabajka Shiinaha. Waxaa la xaqiijiyay markii ugu horeysay in cloconazole ay hoos u dhigtay maadada lignin iyadoo la joojinayo isku-dhafka phenylpropanoid oo ay dhexdhexaadinayaan moduleka BrbZIP39-BrPAL4, taas oo horseedaysa hypocotyl dwarfing ee geedo kaabajka Shiinaha.
Kaabashka Shiinaha (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) waxay ka tirsan tahay cirridka Brassica waana khudradda cruciferous-ka sanadlaha ah ee si weyn looga beero dalkayga (Wang et al., 2022; Yue et al., 2022). Sanadihii la soo dhaafay, miisaanka wax-soo-saarka ee cauliflower Shiinaha ayaa sii waday inuu ballaariyo, habka beerista ayaa ka beddelay abuurka tooska ah ee caadiga ah ilaa dhaqanka seedling degdeg ah iyo tallaalid. Si kastaba ha noqotee, habka dhaqanka seedling degdega ah iyo ku-tallaalidda, korriinka hypocotyl ee xad-dhaafka ah wuxuu u janjeeraa inuu soo saaro geedo leggy, taasoo keentay tayada seedling liidata. Sidaa darteed, xakameynta kobaca hypocotyl-ka xad-dhaafka ah waa arrin culus oo ku saabsan dhaqanka seedling degdega ah iyo tallaalka kaabajka Shiinaha. Waqtigan xaadirka ah, waxaa jira daraasado yar oo isku dhafan xogta transcriptomics iyo metabolomics si loo sahamiyo habka dhererka hypocotyl. Habka molecular ee ay chlorantazole ku maamusho balaadhinta hypocotyl ee kaabajka Shiinaha weli lama baran. Waxaan ujeednay inaan aqoonsanno hidde-sideyaasha iyo waddooyinka molecular ee ka jawaabaya uniconazole-induced hypocotyl dwarfing ee kaabajka Shiinaha. Isticmaalka falanqaynta transcriptome iyo metabolomic, iyo sidoo kale falanqaynta hal-hybrid khamiirka, dual luciferase assay, iyo fayras-ku-soo-celinta hidde-sidaha (VIGS), waxaanu ogaanay in uniconazole uu keeni karo hypocotyl dwarfing ee kaabajka Shiinaha iyada oo la joojinayo lignin biosynthesis ee geedo kaabajka Shiinaha. Natiijooyinkayagu waxay bixiyaan fikrado cusub oo ku saabsan habka nidaaminta molecular kaas oo uniconazole uu u diidayo dhererka hypocotyl ee kaabashka Shiinaha iyada oo la xakameynayo biosynthesis phenylpropanoid oo dhexdhexaadinaysa moduleka BrbZIP39-BrPAL4. Natiijooyinkan ayaa laga yaabaa inay leeyihiin saameyno la taaban karo oo muhiim ah oo lagu hagaajinayo tayada geedo ganacsi iyo ka qayb qaadashada hubinta wax-soo-saarka iyo tayada khudaarta.
Dhererka buuxa ee BrbZIP39 ORF waxaa la geliyey pGreenll 62-SK si loo dhaliyo saamaynta, iyo jajabka dhiirrigeliyaha BrPAL4 waxaa lagu darey hidda-wadaha pGreenll 0800 luciferase (LUC) si loo soo saaro hidda-wadaha wariyaha. Waxyeelaha iyo xididada hidda-wadaha weriyaha ayaa si wadajir ah loogu beddelay caleemaha tubaakada (Nicotiana benthamiana).
Si loo caddeeyo xidhiidhka dheef-shiid kiimikaad iyo hidde-sideyaasha, waxaanu samaynay falanqaynta metabolome-ga wadajirka ah iyo falanqaynta qoraalka. Falanqaynta kobcinta dariiqa KEGG waxay muujisay in DEGs iyo DAMs lagu wada hodmay 33 KEGG (Jaantuska 5A). Waxaa ka mid ah, dariiqa "phenylpropanoid biosynthesis" ayaa ahaa kan ugu aadka u kobcay; Waddada "photosynthetic carbon fixation", waddada "flavonoid biosynthesis", jidka "pentose-glucuronic acid interconversion", dariiqa "metabobolism tryptophan", dariiqa "shiid kiimikaad-sucrose" ayaa sidoo kale si weyn loo kobciyay. Khariidadda ururinta kulaylka (Jaantuska 5B) waxay muujisay in DAM-yada la xidhiidha DEGs loo qaybiyay dhawr qaybood, kuwaas oo flavonoids ay ahaayeen qaybta ugu weyn, taas oo muujinaysa in dariiqa "phenylpropanoid biosynthesis" uu door muhiim ah ka ciyaaray hypocotyl dwarfism.
Qorayaashu waxay caddeeyeen in cilmi-baarista la sameeyay iyada oo aysan jirin wax xiriir ganacsi ama maaliyadeed ah oo loo fasiran karo khilaaf ka iman kara danaha.
Dhammaan ra'yiga lagu sheegay maqaalkan waa kuwa qoraaga oo keliya mana aha inay ka tarjumayaan fikradaha ururrada xiriirka la leh, daabacayaasha, tifaftirayaasha, ama dib-u-eegayaasha. Alaab kasta oo lagu qiimeeyay maqaalkan ama sheegashooyinka ay sameeyeen soosaarayaashoodu ma dammaanad qaadaan ama ma taageeraan daabacuhu.
Waqtiga boostada: Mar-24-2025