Cilmi baadhayaashu waxay ogaadeen sida dhirtu u nidaamiso borotiinka DELLA.

Cilmi-baarayaal ka socda Waaxda Biochemistry ee Machadka Sayniska ee Hindiya (IISc) ayaa helay farsamo muddo dheer la raadinayey.habaynta korriinka dhirta dhulka qadiimiga ah sida bryophytes (koox ay ku jiraan mosses iyo liverworts) kuwaas oo lagu hayo dhirta ubaxyada dambe.
Daraasadda, oo lagu daabacay joornaalka Nature Chemical Biology, ayaa diiradda saarey nidaamka aan kanoniga ahayn ee borotiinka DELLA, maamulaha kobaca sare ee xakameynaya qaybta unugyada ee embryophytes (dhirta dhulka).
Waxa xiiso leh, bryophytes, dhirta ugu horreysa ee ka soo baxda dhulka qiyaastii 500 milyan oo sano ka hor, ayaa ka maqan soo-dhoweynta GID1, inkastoo ay soo saartay phytohormone GA. Tani waxay dhalinaysaa su'aasha ah sida loo habeeyey koritaanka iyo horumarinta dhirtaas hore ee dhulka.
Isticmaalka liverwort Marchantia polymorpha sida nidaamka moodeelka, cilmi-baarayaashu waxay ogaadeen in dhirtan asaasiga ahi ay shaqaaleeyaan enzyme gaar ah, MpVIH, taas oo soo saarta fariinta gacanta inositol pyrophosphate (InsP₈), si ay u burburiso DELLA iyada oo aan ku lug lahayngibberellic acid.
Cilmi-baarayaashu waxay isticmaaleen nidaamka CRISPR-Cas9 si ay u garaacaan hidda-wadaha codeeya enzyme VIH, iyagoo xaqiijinaya doorkeeda. Dhirta aan shaqaynayn VIH waxay soo bandhigtay cillado korriin oo daran iyo cillado aan caadi ahayn, sida caleemaha is haysta, korriinka shucaaca oo daciifa, iyo la'aanta calyxes. Cilladahan ayaa la tirtiray iyadoo la beddelayo genome-ka dhirta si loo soo saaro hal dhammaad (N-terminus) ee enzyme VIH. Iyaga oo isticmaalaya farsamooyinka chromatography ee horumarsan, kooxdu waxay ogaadeen in N-terminus uu ka kooban yahay domain kinase kaas oo kicinaya soo saarista InsP₈.
Cilmi-baarayaashu waxay ogaadeen in DELLA ay tahay mid ka mid ah bartilmaameedyada gacanta ee VIH kinase. Intaa waxaa dheer, waxay arkeen in phenotypes of MpVIH-deficient dhirta ay la mid yihiin kuwa dhirta M. polymorpha oo leh muujinta DELLA oo kordhay.
"Xilligan, waxaan ku faraxsannahay inaan fahamno in xasilloonida DELLA ama dhaqdhaqaaqa lagu wanaajiyey dhirta MpVIH-yar," ayay tiri Priyanshi Rana, qoraaga koowaad iyo arday ka qalin-jabisay kooxda cilmi-baarista Lahey. Iyadoo la raacayo mala-awaalkooda, cilmi-baarayaashu waxay ogaadeen in joojinta DELLA ay si weyn u soo celin karto korriinka cilladaysan iyo noocyada horumarinta ee dhirta mutant ee MpVIH. Natiijooyinkani waxay soo jeedinayaan in VIH kinase ay si xun u maamusho DELLA, kor u qaadida koritaanka iyo horumarka dhirta.
Cilmi-baadhis lagu sameeyay borotiinada DELLA waxay dib ugu soo noqotay Kacaankii Cagaaran, markii saynisyahannadu iyagoon ogayn ay ka faa'iidaysteen awooddooda si ay u abuuraan noocyo-dwarf-sare ah oo wax-soo-saar sare leh. Inkasta oo faahfaahinta shaqadoodu aanay caddayn wakhtigaas, tignoolajiyada casriga ahi waxay u ogolaataa saynisyahannada inay wax ka qabtaan hawlaha borotiinnadan iyada oo loo marayo injineernimada hiddaha, si wax ku ool ah u kordhiya wax-soo-saarka dalagga.