Boqoshaada waaweyn waxay leeyihiin noocyo badan oo kala duwan oo metabolites-ka noolaha firfircoon waxaana loo arkaa ilo-biyood qiimo leh. Phellinus ignarius waa boqoshaada weyn oo dhaqan ahaan loo isticmaalo ujeeddooyin daawo iyo cunto labadaba, laakiin kala-soociddiisa iyo magaca Laatiinka ayaa weli muran ka taagan yahay. Iyagoo adeegsanaya falanqaynta isku-dubaridka qaybaha badan ee hiddo-wadaha, cilmi-baarayaashu waxay xaqiijiyeen in Phellinus ignarius iyo noocyada la midka ah ay ka tirsan yihiin nooc cusub waxayna aasaaseen hidde-side Sanghuangporus. Boqoshaada honeysuckle Sanghuangporus lonicericola waa mid ka mid ah noocyada Sanghuangporus ee la aqoonsaday adduunka oo dhan. Phellinus ignarius waxay soo jiidatay dareen badan sababtoo ah sifooyinkeeda daawada ee kala duwan, oo ay ku jiraan polysaccharides, polyphenols, terpenes, iyo flavonoids. Triterpenes waa isku-dhafka ugu muhiimsan ee firfircoon ee daawada ee hidde-sidedan, iyagoo muujinaya waxqabadyo antioxidant, antibacterial, iyo antitumor.
Triterpenoids waxay leeyihiin awood weyn oo loogu talagalay isticmaalka ganacsiga. Sababtoo ah naadirnimada kheyraadka duurjoogta ah ee Sanghuangporus ee dabiiciga ah, si wax ku ool ah u wanaajinta waxtarkeeda iyo wax soo saarkeeda ayaa muhiimad weyn leh. Waqtigan xaadirka ah, horumar ayaa laga sameeyay kor u qaadista wax soo saarka metabolites-ka kala duwan ee Sanghuangporus iyadoo la adeegsanayo kiimikooyin kiciya si loo xakameeyo istaraatiijiyadaha halsano ee ku jira. Tusaale ahaan, asiidhyada dufanka leh ee polyunsaturated, fangaska elicitors11 iyo phytohormones (oo ay ku jiraan methyl jasmonate iyo salicylic acid14) ayaa la muujiyay inay kordhiyaan wax soo saarka triterpenoid ee Sanghuangporus. Nidaamiyeyaasha koritaanka dhirta(PGR-yada)waxay nidaamin kartaa biosynthesis-ka metabolites-ka labaad ee dhirta. Daraasaddan, waxaa la baaray PBZ, oo ah nidaamiye koritaanka dhirta oo si weyn loogu isticmaalo nidaaminta koritaanka dhirta, wax soo saarka, tayada iyo sifooyinka jir ahaaneed. Gaar ahaan, isticmaalka PBZ wuxuu saameyn ku yeelan karaa waddada biosynthetic-ga terpenoid ee dhirta. Isku darka gibberellins-ka iyo PBZ waxay kordhisay maadada quinone methide triterpene (QT) ee Montevidia floribunda. Halabuurka marinnada terpenoid ee saliidda lavender-ka ayaa la beddelay ka dib markii lagu daaweeyay 400 ppm PBZ. Si kastaba ha ahaatee, ma jiraan warbixino ku saabsan isticmaalka PBZ ee boqoshaada.
Marka laga soo tago daraasadaha diiradda saaraya kororka wax soo saarka triterpene, daraasadaha qaar ayaa sidoo kale caddeeyay hababka nidaaminta ee biosynthesis-ka triterpene ee Moriformis iyadoo ay saameynayaan kiimikooyinka kiciya. Waqtigan xaadirka ah, daraasaduhu waxay diiradda saarayaan isbeddelka heerarka muujinta hidda-wadaha qaab-dhismeedka ee la xiriira biosynthesis-ka triterpene ee marin-haweedka MVA, taas oo horseedaysa kororka wax soo saarka terpenoid.12,14 Si kastaba ha ahaatee, waddooyinka salka ku haya hidda-wadaha qaab-dhismeedka ee la yaqaan, gaar ahaan arrimaha qoraalka ee nidaaminaya muujinta, weli ma cadda hababka nidaaminta ee biosynthesis-ka triterpene ee Moriformis.
Daraasaddan, waxaa la baaray saamaynta kala duwan ee isku-ururinta kala duwan ee nidaamiyayaasha koritaanka dhirta (PGRs) ee wax soo saarka triterpene iyo koritaanka mycelium inta lagu jiro halsano biyaha hoostooda ah ee honeysuckle (S. lonicericola). Intaa ka dib, metabolomics iyo transcriptomics ayaa loo isticmaalay in lagu falanqeeyo halabuurka triterpene iyo qaababka muujinta hidda-wadaha ee ku lug leh biosynthesis-ka triterpene inta lagu jiro daaweynta PBZ. Xogta taxanaha RNA iyo bioinformatics waxay si dheeraad ah u aqoonsatay qodobka qoraalka bartilmaameedka ah ee MYB (SlMYB). Intaa waxaa dheer, isbeddellada ayaa la sameeyay si loo xaqiijiyo saameynta sharciyeed ee hidda-wadaha SlMYB ee biosynthesis-ka triterpene iyo in la aqoonsado hidda-wadaha bartilmaameedka ah ee suurtagalka ah. Baaritaannada isbeddelka dhaqdhaqaaqa elektaroonigga ah (EMSA) ayaa loo isticmaalay in lagu xaqiijiyo isdhexgalka borotiinka SlMYB ee dhiirrigeliyeyaasha hidda-wadaha bartilmaameedka SlMYB. Marka la soo koobo, ujeeddada daraasaddan waxay ahayd in la kiciyo biosynthesis-ka triterpene iyadoo la adeegsanayo PBZ iyo in la aqoonsado qodobka qoraalka MYB (SlMYB) kaas oo si toos ah u nidaamiya hiddo-sidayaasha biosynthetic-ga triterpene oo ay ku jiraan MVD, IDI, iyo FDPS ee S. lonicericola iyadoo laga jawaabayo kicinta PBZ.
Soo saarista labada IAA iyo PBZ waxay si weyn u kordhisay wax soo saarka triterpenoid ee honeysuckle, laakiin saameynta soo saarista PBZ ayaa si aad ah u muuqatay. Sidaa darteed, PBZ waxaa la ogaaday inay tahay soo-saaraha ugu fiican marka la helo 100 mg/L, taasoo u qalanta daraasad dheeraad ah.
Waqtiga boostada: Agoosto-19-2025